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Journal / Genetics / Quantitative Real-Time PCR Evaluation of microRNA …
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JoVE Journal Genetics
Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
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Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Article DOI: 10.3791/61383-v • 07:36 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Summary
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We describe a method for evaluating the microRNA expression in the kidneys of mice with unilateral ureteral obstruction (UUO) by quantitative reverse-transcription polymerase chain reaction. This protocol is suitable for studying kidney microRNA expression profiles in mice with UUO and in the context of other pathological conditions.

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Quantitative Real-Time PCR MicroRNA Expressions Mouse Kidney Unilateral Ureteral Obstruction Renal Fibrosis MicroRNA Expression Profiling Accuracy Sensitivity Simple Process Time-saving Technical Error Prevention Sham Surgery Surgical Scissors Tweezers Incision Skin Muscle Peritoneal Membrane Abdomen Cotton Swabs PBS Left Kidney And Ureter Identification Peritoneal Membrane Closure 4-0 Nylon Sutures Unilateral Urethral Obstruction (UUO) Silk Ligations
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