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JoVE Journal Cancer Research
Övervakning Cancer Cell Invasion och T-Cell cytotoxicitet i 3D-kultur
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Övervakning Cancer Cell Invasion och T-Cell cytotoxicitet i 3D-kultur

Article DOI: 10.3791/61392-v • 07:44 min • June 23rd, 2020
June 23rd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1
1Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University

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Den presenterade metoden utvärderar samtidigt cancer cell invasion i 3D sfäroid analyser och T-cell cytotoxicitet. Sfäroider genereras i en byggnadsställning-fri agaros multi-microwell rösterna. Samkultur och inbäddning i typ I kollagen matris utförs inom samma enhet som gör det möjligt att övervaka cancer cell invasion och T-cells medierad cytotoxicitet.

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Cancerforskning 3D-cellkultur cancerceller samkultur kollagen cytotoxicitet invasion immunofluorescens immunohistokemi t-celler tumörsfäroid
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