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Journal / Developmental Biology / In vitro neuromuskulært veikryss indusert fra…
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Developmental Biology

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JoVE Journal Developmental Biology
In vitro neuromuskulært veikryss indusert fra humane induserte pluripotente stamceller
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In vitro neuromuskulært veikryss indusert fra humane induserte pluripotente stamceller

Article DOI: 10.3791/61396-v • 06:01 min • December 3rd, 2020
December 3rd, 2020 •
1, 1,2, 3, 1
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 2Department of Pediatrics, Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine, Taipei Medical University

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Her gir vi en protokoll for å generere in vitro NMJs fra humane induserte pluripotente stamceller (iPSCer). Denne metoden kan indusere NMJs med moden morfologi og funksjon i 1 måned i en enkelt brønn. De resulterende NMJs kan potensielt brukes til å modellere relaterte sykdommer, for å studere patologiske mekanismer eller å screene narkotikaforbindelser for terapi.

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Utviklingsbiologi utgave 166 iPSCer in vitro nevromuskulært veikryss motorneuron Schwann-celle myotubesammentrekning curare
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