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Immunofluorescens imaging av DNA-skador och reparation foci i mänskliga kolon cancerceller
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Immunofluorescens imaging av DNA-skador och reparation foci i mänskliga kolon cancerceller

Article DOI: 10.3791/61399-v • 05:18 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1, 1
1Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

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Strålningsinducerad DNA-skaderespons som aktiveras av neutronblyglad som används vid behandling med inskiljning av bor neutron (BNCT) har inte fastställts helt. Detta protokoll ger ett steg-för-steg förfarande för att upptäcka strålning-inducerad foci (RIF) av reparation proteiner genom immunofluorescens färgning i mänskliga kolon cancer cellinjer efter bestrålning med neutron-blandade strålen.

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Cancerforskning BNCT DNA-DSBs strålningsinducerade högborgar DNA-skador reparationsvägar blandad stråle
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