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Journal / Immunology and Infection / Neisseria meningitidis Infeksjon av induserte…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Neisseria meningitidis Infeksjon av induserte pluripotente stamcelleavledede hjerneendotelceller
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Neisseria meningitidis Infeksjon av induserte pluripotente stamcelleavledede hjerneendotelceller

Article DOI: 10.3791/61400-v • 10:12 min • July 14th, 2020
July 14th, 2020 •
1, 2, 1, 1,2
1Institute for Hygiene and Microbiology, University of Würzburg, 2Department of Biological Sciences, University of Alabama

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Protokollen beskrevet her fremhever de viktigste trinnene i differensiering av induserte pluripotente stamcelleavledede hjernelignende endotelceller, fremstilling av Neisseria meningitidis for infeksjon og prøveinnsamling for andre molekylære analyser.

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Neisseria meningitidis meningokokkmeningitt hjerneendotelceller vertspatogen-interaksjoner in vivo modellsystemer menneskebasert modell tette veikryss transendotelial elektrisk motstand (TEER) in vitro-modeller primære BEC immortaliserte BEC induserte pluripotente stamceller (iPSCs) IPSC-avledede BEC-er (iPSC-BEC) barriereødeleggelse medfødt immunaktivering bakteriell interaksjon
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