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Journal / Biochemistry / Misurare il legame nucleotidico a proteine di…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Misurare il legame nucleotidico a proteine di membrana intatte e funzionali in tempo reale
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Misurare il legame nucleotidico a proteine di membrana intatte e funzionali in tempo reale

Article DOI: 10.3791/61401-v • 08:33 min • March 11th, 2021
March 11th, 2021 •
1, 1, 1,2
1Department of Physiology, Anatomy and Genetics, University of Oxford, 2Department of Chemistry and Neuroscience Program, Trinity College

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Questo protocollo presenta un metodo per misurare il legame dei nucleotidi adenina ai recettori in tempo reale in un ambiente cellulare. Il legame è misurato come trasferimento di energia di risonanza di Förster (FRET) tra derivati del nucleotide trinitrofenile e proteine marcate con un amminoacido fluorescente non canonico.

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Questo mese in JoVE Numero 169 legame del ligando proteina di membrana canale ionico nucleotide metodo patch clamp fluorescenza spettri recettore
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