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JoVE Journal
Developmental Biology

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基于细胞基和细胞外矩阵的Murine睾丸器官生成
 
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基于细胞基和细胞外矩阵的Murine睾丸器官生成

Article DOI: 10.3791/61403-v 09:18 min October 7th, 2020
October 7th, 2020

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Summary

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Click here for the English version.

在这里,介绍了从原发性新生儿穆林睾丸细胞生成睾丸器官的四种方法,即细胞外基质 (ECM) 和无 ECM 的 2D 和 3D 培养环境。这些技术具有多种研究应用,特别适用于研究睾丸发育和体外生理学。

Tags

发育生物学, 问题 164, 睾丸, 睾酮, 细胞外基质, 2D, 3D, 组装, 管, 内分泌功能
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