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JoVE Journal Neuroscience
Verwendung von primär kultivierten Hippocampus-Neuronen zur Untersuchung der Anordnung von Axon-Anfangssegmenten
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Verwendung von primär kultivierten Hippocampus-Neuronen zur Untersuchung der Anordnung von Axon-Anfangssegmenten

Article DOI: 10.3791/61411-v • 06:53 min • February 12th, 2021
February 12th, 2021 •
1,2,3, 2,3
1School of Basic Medical Sciences, Xi'an Jiaotong University, 2Department of Cell Biology, Duke University, 3Department of Biochemistry, Duke University

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Hier beschrieben wir ein Protokoll zur quantitativen Untersuchung der Anordnung und Struktur der Axon-Anfangssegmente (AIS) von Hippocampus-Neuronen, denen aufgrund des Fehlens eines riesigen Ankyrin-G ein vormontiertes AIS fehlt.

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Retraction Giant ankyrin-G Axon initial segment Primary cultured neurons Transfection Imaging AIS Intensity quantification
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