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Journal / Bioengineering / Methylering Specifikke Multiplex Dråbe PCR ved…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Methylering Specifikke Multiplex Dråbe PCR ved hjælp af Polymer Dråbe Generator Device for hæmatologisk diagnostik
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Methylering Specifikke Multiplex Dråbe PCR ved hjælp af Polymer Dråbe Generator Device for hæmatologisk diagnostik

Article DOI: 10.3791/61421-v • 09:05 min • June 29th, 2020
June 29th, 2020 •
1, 1, 2, 1, 1, 1, 1, 1, 1, 1
1Life Sciences Division, National Research Council of Canada, 2Galenvs Sciences, Inc.

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Epigenetiske markører anvendes til hvide blodlegemer (WBC) subtyping gennem kvantificering af DNA methylering mønstre. Denne protokol præsenterer en multiplex dråbe polymerase kædereaktion (mdPCR) metode ved hjælp af en termoplastisk elastomer (TPE)-baserede microfluidic enhed til dråbe generation giver mulighed for præcise og multiplex methylation-specifikke mål kvantificering af WBC differentialetal.

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Denne måned i JoVE methylering multiplex dråbe PCR hvide blodlegemer subtyping epigenetik dråbe generation termoplastisk elastomer bisulfit behandlet DNA
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