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Isolasjon og kultur av Chick Ciliary Ganglion Neurons
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Isolasjon og kultur av Chick Ciliary Ganglion Neurons

Article DOI: 10.3791/61431-v • 14:36 min • August 8th, 2020
August 8th, 2020 •
*1, *1, 2, 2, 1,2
1Institute of Biomedicine, Department of Medical Sciences - iBiMED, University of Aveiro, 2CNC - Center for Neuroscience and Cell Biology, University of Coimbra
* These authors contributed equally

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Chick ciliary ganglia (CG) er en del av det parasympatiske nervesystemet. Nevronale kulturer av kylling CG nevroner ble vist å være effektive cellemodeller i studiet av nervemuskel interaksjoner. Vi beskriver en detaljert protokoll for disseksjon, dissosiasjon og in vitro kultur av CG nevroner fra chick embryoer.

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Nevrovitenskap Utgave 162 Cellekultur ciliary ganglion kylling embryo disseksjon parasympatiske nevroner nevromuskulære veikryss immunocytokjemi fluorescens mikroskopi
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