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Neuroscience
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Isolation og kultur chick ciliary ganglion neuroner
 
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Isolation og kultur chick ciliary ganglion neuroner

Article DOI: 10.3791/61431-v 14:36 min August 8th, 2020
August 8th, 2020

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Summary

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Chick ciliaere ganglier (CG) er en del af det parasympatiske nervesystem. Neuronal kulturer af chick CG neuroner blev vist sig at være effektive cellemodeller i studiet af nervemuskel interaktioner. Vi beskriver en detaljeret protokol for dissektion, dissociation og in vitro kultur CG neuroner fra kylling embryoner.

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Neurovidenskab Cellekultur ciliaere ganglion chick embryo dissektion parasympatiske neuroner neuromuskulære vejkryds immunokytokemi fluorescensmikroskopi
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