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Bioengineering

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JoVE Journal Bioengineering
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
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Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Article DOI: 10.3791/61433-v • 09:20 min • August 11th, 2020
August 11th, 2020 •
1, 2, 3,4, 2,4, 1
1Department of Mechanical Engineering, University of Kentucky, 2Department of Chemistry, University of Kentucky, 3Department of Physiology, University of Kentucky, 4UK Light Microscopy Core, University of Kentucky

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Presented here is the protocol of the Conpokal technique, which combines confocal and atomic force microscopy into a single instrument platform. Conpokal provides same cell, same region, near simultaneous confocal imaging and mechanical characterization of live biological samples.

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Near Simultaneous Laser Scanning Confocal And Atomic Force Microscopy Conpokal Live Cells Confocal Microscopy Atomic Force Microscopy Fluorescence Co-localization Mechanical Characterization Dual-microscopy Cytoskeletal Processes Cellular Processes AFM Probing Mechanobiology Field Brain Cells Physiological Conditions Electrical Impulses Force Transaction AFM Cantilever AFM Chip Mounting Stage Gloves Tweezers Small Screwdriver Glass Block
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