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Immunology and Infection

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JoVE Journal Immunology and Infection
Beoordeling van de expressie van major histocompatibiliteit complex klasse I op primaire murine hippocampale neuronen door flow cytometrie
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Beoordeling van de expressie van major histocompatibiliteit complex klasse I op primaire murine hippocampale neuronen door flow cytometrie

Article DOI: 10.3791/61436-v • 08:07 min • May 19th, 2020
May 19th, 2020 •
1, 1
1Department of Biological Sciences, University of North Carolina at Charlotte

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Dit protocol beschrijft een methode voor het cultiveren van primaire hippocampale neuronen uit embryonale muishersenen. De expressie van grote histocompatibiliteitscomplex klasse I op het extracellulaire oppervlak van gekweekte neuronen wordt vervolgens beoordeeld door flowcytometrische analyse.

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Immunologie en infectie antigeenpresentatie flowcytometrie hippocampus major histocompatibiliteit complex klasse I microdissection neuro-immuun primaire neuronen
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