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Journal / Genetics / Kultur und Assay von großflächigen Caenorhabditis …
JoVE Journal
Genetics

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JoVE Journal Genetics
Kultur und Assay von großflächigen Caenorhabditis elegans-Populationen im gemischten Stadium
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Kultur und Assay von großflächigen Caenorhabditis elegans-Populationen im gemischten Stadium

Article DOI: 10.3791/61453-v • 08:51 min • May 5th, 2021
May 5th, 2021 •
1, 2, 3, 4, 1,2,3
1Department of Genetics, University of Georgia, 2Department of Biochemistry and Molecular Biology, University of Georgia, 3Complex Carbohydrate Research Center, University of Georgia, 4Department of Molecular Biosciences, Northwestern University

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Um Caenorhabditis elegans (C. elegans) in der Omics-Forschung zu verwenden, wird eine Methode benötigt, um große Populationen von Würmern zu generieren, bei denen eine einzelne Probe plattformübergreifend für vergleichende Analysen gemessen werden kann. Hier wird eine Methode vorgestellt, um C. elegans Populationen auf großräumigen Kulturplatten (LSCPs) zu kulturieren und das Bevölkerungswachstum zu dokumentieren.

Tags

Retraction C. elegans Culturing Large Particle Flow Cytometry Large-Scale Culture Plate Worm Growth Systems Biology Generating Mixed-Stage Cultures Caenorhabditis elegans omics
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