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A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury
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A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

Article DOI: 10.3791/61455-v • 09:50 min • June 17th, 2020
June 17th, 2020 •
*1,2, *1,2, 1, 3,4,5, 1,6, 1,2, 2,7, 2,7, 1,2
1Department of Urology, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School, 3Division of Hematology/Oncology, Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, 7Vascular Biology Program, Boston Children's Hospital
* These authors contributed equally

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Summary
Automatic Translation

The goal of this protocol is to apply an optimized tissue dissociation protocol to a mouse model of spinal cord injury and validate the approach for single cell analysis by flow cytometry.

Tags

Single Cell Dissociation Molecular Analysis Urinary Bladder Mouse Spinal Cord Injury Biological Information High Cell Viability Single Cell Suspension Isolated Cell Types Surgical Procedure Tissue Dissociation Procedure FACS Analysis Anesthetized Mouse Pedal Reflex Spinous Process Analgesia Antibiotics Povidone Iodine Ethanol Wipes Sterile Gauze Sponge Spinal Cord Injury Induction
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