/

/

脊髓损伤后小鼠泌尿膀胱分子分析的单细胞分离方法

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

Please note that all translations are automatically generated. Click here for the English version.

脊髓损伤后小鼠泌尿膀胱分子分析的单细胞分离方法

5,202 Views

•

09:50 min

•

June 17, 2020

DOI:

10.3791/61455-v

DOI:

10.3791/61455-v

•

09:50 min

June 17, 2020

•

5 Views

Hussein Atta*^1,2, Ali Hashemi Gheinani*^1,2, Amanda Wacker¹, Yaser Heshmati^3,4,5, Alex Bigger-Allen^1,6, George Lambrinos^1,2, Yao Gao^2,7, Diane R. Bielenberg^2,7, Rosalyn M. Adam^1,2

¹Department of Urology,Boston Children’s Hospital, ²Department of Surgery,Harvard Medical School, ³Division of Hematology/Oncology,Harvard Medical School Boston, ⁴Dana-Farber Cancer Institute, ⁵Broad Institute, ⁶Biological Biomedical Sciences Program, Division of Medical Sciences,Harvard Medical School, ⁷Vascular Biology Program,Boston Children’s Hospital

Summary

此协议的目标是将优化的组织分离协议应用于脊髓损伤的鼠标模型，并验证通过流细胞学进行单细胞分析的方法。

Explore More Videos

Single Cell Dissociation Molecular Analysis Urinary Bladder Mouse Spinal Cord Injury FACS Analysis Laminectomy Spinal Cord Transection Bladder Expression

Read Article