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Uma única abordagem de dissociação celular para análise molecular da bexiga urinária no rato após lesão medular
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Uma única abordagem de dissociação celular para análise molecular da bexiga urinária no rato após lesão medular

Article DOI: 10.3791/61455-v • 09:50 min • June 17th, 2020
June 17th, 2020 •
*1,2, *1,2, 1, 3,4,5, 1,6, 1,2, 2,7, 2,7, 1,2
1Department of Urology, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School, 3Division of Hematology/Oncology, Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, 7Vascular Biology Program, Boston Children's Hospital
* These authors contributed equally

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O objetivo deste protocolo é aplicar um protocolo otimizado de dissociação tecidual a um modelo de camundongo de lesão medular e validar a abordagem para análise de célula única por citometria de fluxo.

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Biologia Questão 160 Medula Espinhal Lesão célula única dissociação citometria de fluxo murina
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