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Immunology and Infection

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JoVE Journal Immunology and Infection
Stabiliteit en structuur van Vleermuis Major Histocompatibiliteit Complexe Klasse I met Heterologe β2-Microglobuline
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Stabiliteit en structuur van Vleermuis Major Histocompatibiliteit Complexe Klasse I met Heterologe β2-Microglobuline

Article DOI: 10.3791/61462-v • 11:17 min • March 10th, 2021
March 10th, 2021 •
*1,2, *3,4, *2,5, 1,2, 1,2, 2, 2, 4, 1, 4, 1,2
1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences, University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 5Savaid Medical School, University of Chinese Academy of Sciences
* These authors contributed equally

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Het protocol beschrijft experimentele methoden om stabiele grote histocompatibiliteitscomplexen (MHC) klasse I te verkrijgen door middel van mogelijke β2-microglobuline (β2m) vervangingen van verschillende soorten. De structurele vergelijking van MHC I gestabiliseerd door homologe en heterologe β2m werd onderzocht.

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Immunologie en infectie Major histocompatibility antigen complex (MHC) klasse I moleculen β2-microglobuline (β2m) complexe structuur peptide TCR tetramer
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