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Journal / Immunology and Infection / Analisi del metabolismo delle cellule…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analisi del metabolismo delle cellule dell'assassino naturale umano
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Analisi del metabolismo delle cellule dell'assassino naturale umano

Article DOI: 10.3791/61466-v • 09:03 min • June 22nd, 2020
June 22nd, 2020 •
1,2, 3, 3
1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

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In questo articolo, descriviamo un metodo per misurare la glicolysi e la respirazione mitocondriale nelle cellule dell'assassino naturale umano primario (NK) isolate dal sangue periferico, a riposo o dopo l'attivazione indotta da IL15. Il protocollo descritto potrebbe essere facilmente esteso alle cellule NK umane primarie attivate da altre citochine o stimoli solubili.

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Immunologia e infezione Problema 160 Cellule Killer Naturali metabolismo flusso extracellulare glicolisi mitocondri respirazione catena di trasporto elettronico citometria di flusso
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