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Journal / Immunology and Infection / Analys av människans naturliga Killer Cell…
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Immunology and Infection

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Analys av människans naturliga Killer Cell Metabolism
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Analys av människans naturliga Killer Cell Metabolism

Article DOI: 10.3791/61466-v • 09:03 min • June 22nd, 2020
June 22nd, 2020 •
1,2, 3, 3
1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

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I detta dokument beskriver vi en metod för att mäta glykolys och mitokondriell respiration i primära mänskliga Natural Killer (NK) celler isolerats från perifert blod, i vila eller efter IL15-inducerad aktivering. Det protokoll som beskrivs skulle lätt kunna utvidgas till primära mänskliga NK celler aktiveras av andra cytokiner eller lösliga stimuli.

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Immunologi och infektion Natural Killer celler metabolism extracellulära fluss glykolys mitokondrier respiration elektrontransportkedjan flödescytometri
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