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Journal / Immunology and Infection / İnsan Doğal Katil Hücre Metabolizmasının…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
İnsan Doğal Katil Hücre Metabolizmasının Analizi
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
Journal (Immunology and Infection)
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore
Journal (Immunology and Infection)
Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore
 
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İnsan Doğal Katil Hücre Metabolizmasının Analizi

Article DOI: 10.3791/61466-v • 09:03 min • June 22nd, 2020
June 22nd, 2020 •
1,2, 3, 3
1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

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Bu yazıda, periferik kandan izole edilen primer insan Doğal Öldürücü (NK) hücrelerinde glikoliz ve mitokondriyal solunumu ölçmek için bir yöntem, istirahatte veya IL15 kaynaklı aktivasyonu takiben açıklıyoruz. Açıklanan protokol kolayca birincil insan NK hücreleri diğer sitokinler veya çözünür uyaranlar tarafından aktive genişletilebilir.

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İmmünoloji ve Enfeksiyon Sayı 160 Doğal Öldürücü hücreler metabolizma hücre dışı akı glikoliz mitokondri solunum elektron taşıma zinciri akış sitometrisi
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