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Journal / Cancer Research / Isolering af humane perifere blodmononukleære…
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Cancer Research

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JoVE Journal Cancer Research
Isolering af humane perifere blodmononukleære celler og CD4 + T-celler fra Sézary syndrom patienter til transkriptomisk profilering
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Isolering af humane perifere blodmononukleære celler og CD4 + T-celler fra Sézary syndrom patienter til transkriptomisk profilering

Article DOI: 10.3791/61470-v • 09:08 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1
1Department of Dermatology, University of Arkansas for Medical Sciences

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Vi præsenterer en simpel protokol til isolering af mononukleære celler i perifert blod fra fuldblod opnået fra patienter diagnosticeret med Sézary syndrom efterfulgt af udvælgelse af CD4 + T-celler, deres stimulering med phorbol12-myristate13-acetat og A23187 ionophore og forberedelse af RNA til transkriptomisk profilering.

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Kræftforskning udgave 176 kutant T-cellelymfom Sézary syndrom mononukleær celle i perifert blod phorbol-12-myrisat13-acetat A23187 mikroarray progression neoplasma.
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