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Journal / Cancer Research / Isolando células mononucleares de sangue…
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Cancer Research

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JoVE Journal Cancer Research
Isolando células mononucleares de sangue periféricos humanos e células T CD4+ de pacientes com síndrome de Sézary para perfil transcriômico
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Isolando células mononucleares de sangue periféricos humanos e células T CD4+ de pacientes com síndrome de Sézary para perfil transcriômico

Article DOI: 10.3791/61470-v • 09:08 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1
1Department of Dermatology, University of Arkansas for Medical Sciences

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Apresentamos um protocolo simples para o isolamento de células mononucleares de sangue periférico de sangue inteiro obtidos de pacientes diagnosticados com Síndrome de Sézary, seguido pela seleção de células T CD4+, sua estimulação com phorbol12-myristate13-acetato e ionóforo A23187, e preparação de RNA para perfil transcriômico.

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Cancer Research Edição 176 linfoma cutâneo de células T síndrome de Sézary célula mononuclear de sangue periférico phorbol-12-myrisate13-acetato A23187 microarray progressão neoplasia.
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