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JoVE Journal
Biology

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使用洗涤剂和酶无酶方法从整个组织进行核分离
 
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使用洗涤剂和酶无酶方法从整个组织进行核分离

Article DOI: 10.3791/61471-v
June 24th, 2020

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Summary June 24th, 2020

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Click here for the English version.

单核分离依赖于细胞膜的分离和基于洗涤剂的渗透,这些步骤需要优化,并且容易引入技术伪影。我们演示了一种洗涤剂和无酶协议,用于直接从整个组织中快速分离完整的核,产生适合单核RNA-seq(snRNA-Seq)或 ATAC-seq 的核。

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