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Journal / Environment / RNA interferens i akvatiske biller som et…
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Environment

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JoVE Journal Environment
RNA interferens i akvatiske biller som et effektivt værktøj til at manipulere genekspression på specifikke udviklingsmæssige tidspunkter
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RNA interferens i akvatiske biller som et effektivt værktøj til at manipulere genekspression på specifikke udviklingsmæssige tidspunkter

Article DOI: 10.3791/61477-v • 08:55 min • May 29th, 2020
May 29th, 2020 •
1, 1, 2,3, 1,4, 2, 1
1Department of Biological Sciences, University of Cincinnati, 2Department of Biology, Miami University, 3Department of Biology, University of North Carolina at Chapel Hill, 4Department of Neuroscience, The Scripps Research Institute

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RNA-interferens er en bredt anvendelig, kraftfuld teknik til at manipulere genekspression på bestemte udviklingsstadier. Her beskriver vi de nødvendige skridt til at gennemføre denne teknik i den akvatiske dykning bille Thermonectus marmoratus, fra erhvervelse af gensekvenser til knockdown af gener, der påvirker struktur eller adfærd.

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Miljøvidenskab Problem 159 RNA-interferens dobbeltstrenget RNA dsRNA insekter funktionstab bille
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