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Påvisning av Phytophthora capsici i vanning vanning vann ved hjelp av Loop-mediert isotermisk forsterkning
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Påvisning av Phytophthora capsici i vanning vanning vann ved hjelp av Loop-mediert isotermisk forsterkning

Article DOI: 10.3791/61478-v • 07:25 min • June 25th, 2020
June 25th, 2020 •
1, 1, 2, 3, 4, 1
1Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center, University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University

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Vi utviklet en metode for å oppdage Phytophthora capsici zoospores i vannkilder ved hjelp av en filterpapir DNA ekstraksjon metode kombinert med en loop-mediert isothermal forsterkning (LAMP) analyse som kan analyseres i feltet eller i laboratoriet.

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Denne måneden i JoVE Utgave 160 Phytophthora capsici Vanning vann Zoospore deteksjon LAMP analyse Filterpapir Rask deteksjon DNA ekstraksjon In-field diagnose
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