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Biochemistry

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JoVE Journal Biochemistry
Strategia fosfoproteomica per la profilazione del segnale di stress osmotico in Arabidopsis
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Strategia fosfoproteomica per la profilazione del segnale di stress osmotico in Arabidopsis

Article DOI: 10.3791/61489-v • 05:47 min • June 25th, 2020
June 25th, 2020 •
1,3, 2, 3,4, 5
1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences

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Qui è presentato un approccio fosfoproteomico, vale a dire fosfoproteomico a base di punta di estrazione stop and go, che fornisce un'elevata produttività e una copertura profonda del fosfoproteoma arabidopsis. Questo approccio delinea la panoramica della segnalazione dello stress osmotico in Arabidopsis.

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Biochimica Numero 160 spettrometria di massa fosfoproteomica fosforilazione proteica approccio alle punte dello stadio etichettatura tandem mass tag arabidopsis,stress osmotico
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