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JoVE Journal
Neuroscience

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通过特洛多毒素微注射可逆灭活,揭开鼠脑离散区在排卵调节中的作用
 
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通过特洛多毒素微注射可逆灭活,揭开鼠脑离散区在排卵调节中的作用

Article DOI: 10.3791/61493-v 11:04 min September 3rd, 2020
September 3rd, 2020

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Summary

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本协议描述了低成本微注射系统的构建、对深脑结构的立体定向植入以及清醒和无拘无束的大鼠对四毒素定时微注射的程序。目标是通过抑制下丘脑结构的神经活动来揭示下丘脑结构参与排卵的调节。

Tags

神经科学, 第 163 期, 内脑显微注射, 立体定型手术, 四毒素, 排卵, 雌激素周期, 神经内分泌学
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