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Journal / Neuroscience / Optrævling af de diskrete områder af rottehjernen …
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JoVE Journal Neuroscience
Optrævling af de diskrete områder af rottehjernen i reguleringen af ægløsning gennem reversibel inaktivering af Tetrodotoxin Microinjections
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Optrævling af de diskrete områder af rottehjernen i reguleringen af ægløsning gennem reversibel inaktivering af Tetrodotoxin Microinjections

Article DOI: 10.3791/61493-v • 11:04 min • September 3rd, 2020
September 3rd, 2020 •
*1,2, *1, 4, 1,2, 3,5, 3, 1,2,3
1Chronobiology of Reproduction Research Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 2Developmental Biology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 3Neuroendocrinology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, 4Facultad de Ciencias, Universidad Nacional Autónoma de México, 5Department of Basic Research, Instituto Nacional de Geriatria, Ministry of Health
* These authors contributed equally

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Denne protokol beskriver opførelsen af et billigt mikroinjektionssystem, dets stereotaxiske implantation i dybhjernestrukturer og proceduren for tidsurige mikroinjektioner af tetrodotoksin hos vågne og hæmningsløse rotter. Målet er at afsløre deltagelsen af hypothalamus strukturer i reguleringen af ægløsning ved at hæmme deres neurale aktivitet.

Tags

Neurovidenskab Udgave 163 Intracerebral mikroinjektion Stereotaxisk kirurgi Tetrodotoxin Ægløsning Estrous cyklus Neuroendocrinology
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