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En Suppressor Skærm til karakterisering af genetiske links regulering kronologisk levetid i Saccharomyces cerevisiae
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En Suppressor Skærm til karakterisering af genetiske links regulering kronologisk levetid i Saccharomyces cerevisiae

Article DOI: 10.3791/61506-v • 10:39 min • September 17th, 2020
September 17th, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biology, William Paterson University

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Her er en protokol til at identificere genetiske interaktioner gennem en øget kopi nummer suppressor skærm i Saccharomyces cerevisiae. Denne metode gør det muligt for forskere at identificere, klone og testsup suppressorer i kortlivede gærmutanter. Vi tester effekten af kopinummerets stigning af SIR2 på levetiden i en autofagi null mutant.

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Genetik kronologisk aldring autofagi SIR2 lysin deacetylase suppressor skærm Saccharomyces cerevisiae kopi nummer skærm
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