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Journal / Genetics / En suppressor skjerm for karakterisering av…
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JoVE Journal Genetics
En suppressor skjerm for karakterisering av genetiske koblinger regulere kronologisk levetid i saccharomyces cerevisiae
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En suppressor skjerm for karakterisering av genetiske koblinger regulere kronologisk levetid i saccharomyces cerevisiae

Article DOI: 10.3791/61506-v • 10:39 min • September 17th, 2020
September 17th, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biology, William Paterson University

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Her er en protokoll for å identifisere genetiske interaksjoner gjennom en økt kopi nummer suppressor skjerm i Saccharomyces cerevisiae. Denne metoden gjør det mulig for forskere å identifisere, klone og teste suppressors i kortvarige gjærmutanter. Vi tester effekten av kopinummerøkningen av SIR2 på levetid i en autofagi null mutant.

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Genetikk Utgave 163 kronologisk aldring autofagi SIR2 lysine deacetylase suppressor skjerm Saccharomyces cerevisiae kopi nummer skjerm
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