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JoVE Journal Medicine
Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development
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Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

Article DOI: 10.3791/61516-v • 08:07 min • July 1st, 2020
July 1st, 2020 •
1,2, 1,2, 3, 1,2,4
1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", 2IRCCS Santa Lucia Foundation, 3Department of Biology and Biotechnology, "C. Darwin" Sapienza University, CNR - IBPM, 4CNR – Institute of Biomedical Technologies (ITB)

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Hier schlagen wir eine Methode vor, um einzelne Muskelfasern in frühen postnatalen Entwicklungsstadien aus dem homozygoten Mutanten Lamin-Mausmodell, einem sehr schweren Modell für Emery-Dreifuss-Muskeldystrophie (EDMD), effizient zu erhalten.

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Medizin Ausgabe 161 Emery-Dreifuss Muskeldystrophie Mausmodell Myofiber Immunfluoreszenz Satellitenmuskelstammzellen Differenzierung Muskelregeneration
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