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激光捕获微分块RNA-测序空间和时间组织特异性基因表达分析
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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
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激光捕获微分块RNA-测序空间和时间组织特异性基因表达分析

English

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7,666 Views

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08:33 min

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August 05, 2020

DOI:

10.3791/61517-v

DOI:
10.3791/61517-v

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08:33 min
August 05, 2020

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6 Views
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1Department of Animal, Plant and Soil Science, AgriBio Building,La Trobe University, 2Australian Research Council Centre of Excellence in Plant Energy Biology,La Trobe University, 3Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building,La Trobe University

Summary

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这里介绍的是植物组织激光捕获微切除（LCM）的协议。LCM 是一种以无污染方式隔离组织区域的微观技术。该过程包括组织固定、石蜡嵌入、分切、LCM和RNA提取。RNA用于下游组织特异性，时间解析的转录组分析。

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Cite this Article

Liew, L. C., Wang, Y., Peirats-Llobet, M., Berkowitz, O., Whelan, J., Lewsey, M. G. Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants. J. Vis. Exp. (162), e61517, doi:10.3791/61517 (2020).

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