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Journal / Biology / Laserfangst mikrodisseksjon RNA-sekvensering for…
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Laserfangst mikrodisseksjon RNA-sekvensering for romlig og temporal vevspesifikt genuttrykksanalyse i planter
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Laserfangst mikrodisseksjon RNA-sekvensering for romlig og temporal vevspesifikt genuttrykksanalyse i planter

Article DOI: 10.3791/61517-v • 08:33 min • August 5th, 2020
August 5th, 2020 •
1,2, 1,2, 1, 1,2,3, 1,2,3, 1,3
1Department of Animal, Plant and Soil Science, AgriBio Building, La Trobe University, 2Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, 3Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building, La Trobe University

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Presentert her er en protokoll for laser-fangst mikrodisseksjon (LCM) av plantevev. LCM er en mikroskopisk teknikk for å isolere områder av vev på en forurensningsfri måte. Prosedyren inkluderer vev fiksering, parafin innebygging, snitting, LCM og RNA ekstraksjon. RNA brukes i nedstrøms vevsspesifikke, tidsmessig løste analyser av transkripsjoner.

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Biologi utgave 162 laserfangst mikrodisseksjon LCM transkripsjon vevsspesifikk RNA-seq genuttrykk romlig
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