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Journal / Biology / Sondering av mRNA Kinetikk i rom og tid i…
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Biology

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JoVE Journal Biology
Sondering av mRNA Kinetikk i rom og tid i Escherichia coli ved hjelp av to-farge enkeltmolekyl fluorescens i Situ hybridisering
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Sondering av mRNA Kinetikk i rom og tid i Escherichia coli ved hjelp av to-farge enkeltmolekyl fluorescens i Situ hybridisering

Article DOI: 10.3791/61520-v • 10:01 min • July 30th, 2020
July 30th, 2020 •
1,2,3,4, 1,2
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

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Denne protokollen beskriver en anvendelse av enkeltmolekylfluorescens in situ hybridisering (smFISH) for å måle in vivo kinetikk av mRNA syntese og nedbrytning.

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Biologi Utgave 161 smFISH RNA-avbildning mRNA lokalisering enkeltcelle enkeltmolekyl transkripsjon transkripsjon forlengelse tidlig avslutning mRNA nedbrytning
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