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Journal / Biology / Sondare la cinetica dell'mRNA nello spazio e nel…
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Biology

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Sondare la cinetica dell'mRNA nello spazio e nel tempo in Escherichia coli utilizzando la fluorescenza a singola molecola a due colori in ibridazione situ

Article DOI: 10.3791/61520-v • 10:01 min • July 30th, 2020
July 30th, 2020 •
1,2,3,4, 1,2
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

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Questo protocollo descrive un'applicazione di fluorescenza a singola molecola in ibridazione situ (smFISH) per misurare la cinetica in vivo della sintesi e della degradazione dell'mRNA.

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Biologia Problema 161 smFISH imaging dell'RNA localizzazione dell'mRNA singola cellula singola molecola trascrizione allungamento della trascrizione terminazione prematura degradazione dell'mRNA
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