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Indringende mRNA Kinetics in ruimte en tijd in Escherichia coli met behulp van Two-Color Single-Molecule Fluorescentie in Situ Hybridisatie
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Indringende mRNA Kinetics in ruimte en tijd in Escherichia coli met behulp van Two-Color Single-Molecule Fluorescentie in Situ Hybridisatie

Article DOI: 10.3791/61520-v • 10:01 min • July 30th, 2020
July 30th, 2020 •
1,2,3,4, 1,2
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

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Dit protocol beschrijft een toepassing van fluorescentie met één molecuul in situ hybridisatie (smFISH) om de in vivo kinetiek van mRNA-synthese en -afbraak te meten.

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Biologie smFISH RNA imaging mRNA lokalisatie eencellige enkele molecuul transcriptie transcriptieverlenging voortijdige beëindiging mRNA afbraak
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