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Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
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Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring

Article DOI: 10.3791/61525-v • 09:34 min • August 6th, 2020
August 6th, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 1, 2, 1,3
1Genome Biology of Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), 2Applied Genomics for Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), 3Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research, University of Tübingen
* These authors contributed equally

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Summary
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We show the automation of human induced pluripotent stem cell (hiPSC) cultures and neuronal differentiations compatible with automated imaging and analysis.

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Automated Production Human Induced Pluripotent Stem Cells Cortical Neurons Dopaminergic Neurons Live-cell Monitoring Automated Procedures Culturing Differentiating Neuronal Lineages Imaging Experimental Variation Phenotypic Reproducibility Protocols Development Graphic User Interface Resource Instrument Process View Run Instrument Process HEPA Hood Reloading Processes Cell Culture Plates Disposable Tips Decontamination Process Sterilization With UV Radiation Culture Medium Dissociation Reagent
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