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Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

Article DOI: 10.3791/61532-v • 08:58 min • August 25th, 2020
August 25th, 2020 •
*1, *1, 1, 1, 1, 1, 2, 1
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology, 2The 2nd Dental Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology
* These authors contributed equally

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Summary
Automatic Translation

This article presents a method that combines whole bone marrow adherence and flow cytometry sorting for isolating, cultivating, sorting, and identifying bone marrow mesenchymal stem cells from rat mandibles.

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Isolation Cultivation Mandibular Bone Marrow Mesenchymal Stem Cells Rats Craniofacial Therapeutics Protocol Whole Bone Marrow Adherence Fluorescent Cell Sorting Purified Anatomic Structure Functional Movement Rat Mandible Euthanizing Fume Hood Supine Position Incise Skin Buccinator Muscles Angulus Oris Posterior Region Mandibular Teeth Buccal Muscles Tendons Coracoids Inferior Border Condyles Mandible Body Skull
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