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Isolering og berigelse af humane lungeepitelceller til organoidkultur
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Isolering og berigelse af humane lungeepitelceller til organoidkultur

Article DOI: 10.3791/61541-v • 11:49 min • July 21st, 2020
July 21st, 2020 •
1, 1, 1, 1, 1, 1
1Lung and Regenerative Medicine Institutes, Department of Medicine, Cedars-Sinai Medical Center

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Denne artikel indeholder en detaljeret metode til vævsdissociation og cellulære fraktioneringsmetoder, der muliggør berigelse af levedygtige epitelceller fra proksimale og distale regioner i den menneskelige lunge. Heri anvendes disse tilgange til funktionel analyse af lungeepitelprogenitorceller ved brug af 3D-organoidkulturmodeller.

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Biologi udgave 161 lunge epitel organoidkultur sygdomsmodellering alveolære type II-celler FACS
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