Three-Dimensional Motor Nerve Organoid Generation

Tatsuya Osaki; Siu Yu  A. Chow; Yui Nakanishi; Joel Hern&#225;ndez; Jiro Kawada; Teruo Fujii; Yoshiho Ikeuchi

doi:10.3791/61544

Journal

/

Neuroscience

/

Generazione organoide nervosa motoria tridimensionale

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Three-Dimensional Motor Nerve Organoid Generation

Generazione organoide nervosa motoria tridimensionale

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8,932 Views

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09:57 min

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September 24, 2020

DOI:

10.3791/61544-v

DOI:

10.3791/61544-v

•

09:57 min

September 24, 2020

•

34 Views

Tatsuya Osaki^1,2, Siu Yu A. Chow^1,2, Yui Nakanishi^1,2, Joel Hernández^1,3, Jiro Kawada⁴, Teruo Fujii¹, Yoshiho Ikeuchi^1,2

¹Institute of Industrial Science,The University of Tokyo, ²Department of Chemistry and Biotechnology, School of Engineering,The University of Tokyo, ³Faculty of Science and Engineering,Tecnologico de Monterrey, ⁴Jiksak Bioengineering, Inc.

Transcript

Summary

Questo protocollo fornisce una procedura completa per fabbricare organoide del nervo motorio derivato dalle cellule iPS umane attraverso l'assemblaggio spontaneo di un robusto fascio di assoni estesi da uno sferoide in un chip di coltura tissutale.

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Generazione organoide nervosa motoria tridimensionale

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