Three-Dimensional Motor Nerve Organoid Generation

Tatsuya Osaki; Siu Yu  A. Chow; Yui Nakanishi; Joel Hern&#225;ndez; Jiro Kawada; Teruo Fujii; Yoshiho Ikeuchi

doi:10.3791/61544

Journal

/

Neuroscience

/

Geração organoide do nervo motor tridimensional

Three-Dimensional Motor Nerve Organoid Generation

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Three-Dimensional Motor Nerve Organoid Generation

Geração organoide do nervo motor tridimensional

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8,975 Views

•

09:57 min

•

September 24, 2020

DOI:

10.3791/61544-v

DOI:

10.3791/61544-v

•

09:57 min

September 24, 2020

•

34 Views

Tatsuya Osaki^1,2, Siu Yu A. Chow^1,2, Yui Nakanishi^1,2, Joel Hernández^1,3, Jiro Kawada⁴, Teruo Fujii¹, Yoshiho Ikeuchi^1,2

¹Institute of Industrial Science,The University of Tokyo, ²Department of Chemistry and Biotechnology, School of Engineering,The University of Tokyo, ³Faculty of Science and Engineering,Tecnologico de Monterrey, ⁴Jiksak Bioengineering, Inc.

Transcript

Summary

Este protocolo fornece um procedimento abrangente para fabricar organoide nervoso motor derivado de células iPS humanas através da montagem espontânea de um robusto feixe de axônios estendidos de um esferoide em um chip de cultura tecidual.

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Geração organoide do nervo motor tridimensional

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