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JoVE Journal Cancer Research
使用 CRISPR 介质基础编辑器对 BRCA1 变体进行功能评估
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使用 CRISPR 介质基础编辑器对 BRCA1 变体进行功能评估

Article DOI: 10.3791/61557-v • 09:22 min • February 28th, 2021
February 28th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Department of Biomedical Sciences, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, 2Stem Cell Immunomodulation Research Center, University of Ulsan College of Medicine

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BRCA1突变患者患癌症的风险较高，这值得准确评估BRCA1变异的功能。在此，我们描述了使用CRISPR介质细胞素基础编辑器对BRCA1变种进行功能评估的协议，该基编辑器支持活细胞中目标C：G到T：A的转换。

Tags

癌症研究， 问题 168， CRISPR-Cas， 基础编辑， BE3， BRCA1， 功能评估， 基因组工程
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