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Journal / Medicine / Akut nyreskade model induceret af Cisplatin hos…
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Akut nyreskade model induceret af Cisplatin hos voksne zebrafisk
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Journal (Medicine)
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Assessing Phagocytic Clearance of Cell Death in Experimental Stroke by Ligatable Fluorescent Probes
Journal (Medicine)
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Akut nyreskade model induceret af Cisplatin hos voksne zebrafisk

Article DOI: 10.3791/61575-v • 13:25 min • May 15th, 2021
May 15th, 2021 •
*1, *1, *1,2, 1, 1, 1, 1,2
1Department of Immunology, University of São Paulo, 2Department of Medicine, Nephrology Division, Federal University of São Paulo
* These authors contributed equally

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Denne protokol beskriver procedurerne for at fremkalde akut nyreskade (AKI) hos voksne zebrafisk ved hjælp af cisplatin som et nefrotoksisk middel. Vi detaljerede trin til at evaluere reproducerbarheden af teknikken og to teknikker til at analysere inflammation og celledød i henholdsvis nyrevævet, flowcytometri og TUNEL.

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Medicin Nyre Akut nyreskade (AKI) Cisplatin Zebrafish Inflammation Flow Cytometry TUNEL Cell Death
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