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Journal / Biochemistry / Analyse van SEC-SAXS-gegevens via…
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Biochemistry

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JoVE Journal Biochemistry
Analyse van SEC-SAXS-gegevens via EFA-deconvolution en Scatter
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Analyse van SEC-SAXS-gegevens via EFA-deconvolution en Scatter

Article DOI: 10.3791/61578-v • 10:59 min • January 28th, 2021
January 28th, 2021 •
*1, 2, 3, *4
1European Synchrotron Radiation Facility Structural Biology Group, Structural Biology Group, 2Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 3Diamond Light Source, 4Laboratoire de Physiologie Cellulaire and Végétale, Université Grenoble Alpes/CNRS/CEA/INRA/BIG
* These authors contributed equally

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SEC-BioSAXS metingen van biologische macromoleculen zijn een standaardbenadering voor het bepalen van de oplossingsstructuur van macromoleculen en hun complexen. Hier analyseren we SEC-BioSAXS-gegevens van twee soorten vaak aangetroffen SEC-sporen: chromatogrammen met volledig opgeloste en gedeeltelijk opgeloste pieken. We tonen de analyse en deconvolutie met behulp van scatter en BioXTAS RAW.

Tags

Biochemie Bio-Small Angle X-ray Scattering BioSAXS inline size-exclusion chromatografie SEC achtergrondaftrekking E9 vaccinia Scatter deconvolutie van SEC-SAXS data BioXTAS RAW Inline size-exclusion chromatografie in combinatie met biologische kleine hoek x-ray verstrooiing (SEC-BioSAX)
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