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JoVE Journal
Biochemistry

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实现蛋白质快速光化学氧化的实时补偿,用于确定蛋白质地形变化
 
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实现蛋白质快速光化学氧化的实时补偿,用于确定蛋白质地形变化

Article DOI: 10.3791/61580
September 1st, 2020

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Summary September 1st, 2020

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蛋白质的快速光化学氧化是蛋白质结构表征的新兴技术。不同的溶剂添加剂和配体具有不同的羟基基清除特性。为了比较不同条件下的蛋白质结构,需要实时补偿反应中产生的羟基基,使反应条件正常化。

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