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Bioengineering

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JoVE Journal Bioengineering
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
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Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Article DOI: 10.3791/61585-v • 08:44 min • July 30th, 2020
July 30th, 2020 •
1,2, 1,3, 1,3
1Robert M. Berne Cardiovascular Research Center, University of Virginia, 2Department of Pathology, University of Virginia, 3Department of Medicine, Division of Cardiovascular Medicine, University of Virginia

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Summary
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The goal of this protocol is to synthesize fluorescently-labeled liposomes and use flow cytometry to identify in vivo localization of liposomes at a cellular level.

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Preparation Administration Assessment In Vivo Tissue-specific Cellular Uptake Fluorescent Dye-labeled Liposomes Protocol Tesaglitazar Unbiased Method Low Temperatures Easy To Acquire Equipment Emulsify Sonication Rotary Evaporator Vacuum Line Organic Solvents Gel Formation Filter Liposome Aqueous Dispersion Size Distribution Dynamic Light Scattering Spin Column Unentrapped Tesaglitazar Spectrophotometer
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