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Journal / Immunology and Infection / Конструирование мутантов серотипа 1 Streptococcus …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Конструирование мутантов серотипа 1 Streptococcus pneumoniae штамма 519/43
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Конструирование мутантов серотипа 1 Streptococcus pneumoniae штамма 519/43

Article DOI: 10.3791/61594-v • 06:06 min • September 11th, 2020
September 11th, 2020 •
1, 2, 3, 2, 1
1Faculty of Infections and Tropical Diseases, Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Division of Medicine, University College London, 3Division of Infection and Immunity, University College London UCL

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Здесь мы описываем штамм S. pneumoniae серотипа 1 519/43, который может быть генетически модифицирован, используя его способность естественным образом приобретать ДНК и плазмиду самоубийства. В качестве доказательства принципа был получен изогенный мутант в гене пневмолизина (ply).

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Иммунология и инфекция выпуск 163 серотип 1 S. pneumoniae мутагенез естественная компетентность менингитный пояс
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