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Journal / Bioengineering / Manipulatie van enkele neurale stamcellen en…
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Bioengineering

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JoVE Journal Bioengineering
Manipulatie van enkele neurale stamcellen en neuronen in hersenschijfjes met behulp van roboticamicroïsjectie
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Manipulatie van enkele neurale stamcellen en neuronen in hersenschijfjes met behulp van roboticamicroïsjectie

Article DOI: 10.3791/61599-v • 11:40 min • January 21st, 2021
January 21st, 2021 •
*1,2, *3, 3, 3,4, 1,5,6
1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota
* These authors contributed equally

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Dit protocol toont het gebruik van een robotplatform voor micro-injectie in enkele neurale stamcellen en neuronen in hersenschijfjes. Deze techniek is veelzijdig en biedt een methode om cellen te volgen in weefsel met een hoge ruimtelijke resolutie.

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Bio-engineering hersenontwikkeling stamcel lijntracering robotica neuron microinjectie organotypic weefsel slice eencellige manipulatie
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