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Journal / Bioengineering / Manipulación de células madre y neuronas…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Manipulación de células madre y neuronas neuronales únicas en las rebanadas cerebrales mediante la microinyección robótica
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Manipulación de células madre y neuronas neuronales únicas en las rebanadas cerebrales mediante la microinyección robótica

Article DOI: 10.3791/61599-v • 11:40 min • January 21st, 2021
January 21st, 2021 •
*1,2, *3, 3, 3,4, 1,5,6
1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota
* These authors contributed equally

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Este protocolo demuestra el uso de una plataforma robótica para la microinyección en células madre y neuronas neuronales individuales en las rebanadas cerebrales. Esta técnica es versátil y ofrece un método de seguimiento de células en tejido con alta resolución espacial.

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Bioingeniería Número 167 desarrollo cerebral células madre trazado de linajes robótica neurona microinyección rebanada de tejido organotípico manipulación de una sola célula
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